Gender differences in blood pressure, renal function and response to a high-sodium diet in Wistar rats

  • Luis A. Di Ciano Laboratorio de Riñón Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires (CONICET-UBA), Buenos Aires
  • Pablo J. Azurmendi Laboratorio de Riñón Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires (CONICET-UBA), Buenos Aires
  • Sandra G. Vlachovsky Laboratorio de Riñón Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires (CONICET-UBA), Buenos Aires
  • Elisabet M. Oddo Laboratorio de Riñón Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires (CONICET-UBA), Buenos Aires
  • Elvira Arrizurieta Laboratorio de Riñón Experimental y Bioquímica Molecular, Instituto de Investigaciones Médicas Alfredo Lanari, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad de Buenos Aires (CONICET-UBA), Buenos Aires
  • Claudia M. Silberstein Departamento de Ciencias Fisiológicas, Instituto de Fisiología y Biofísica Bernardo Houssay, Consejo Nacional de Investigaciones Científicas y Técnicas (IFIBIO Houssay-CONICET), Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires
  • Fernando R. Ibarra Departamento de Ciencias Fisiológicas, Instituto de Fisiología y Biofísica Bernardo Houssay, Consejo Nacional de Investigaciones Científicas y Técnicas (IFIBIO Houssay-CONICET), Facultad de Medicina, Universidad de Buenos Aires (UBA), Buenos Aires
Keywords: sex, gender differences, sodium balance, blood pressure, sodium transporters, sodium, Wistar rats, metabolic diseases

Abstract

Introduction: It is known that sex is a determinant of renal sodium regulation and blood pressure. Methods: Male and female Wistar rats, which were 150 days old and a diet with normal or high levels of sodium (NaCl 1% v.o.), were studied for the last five days. Mean blood pressure (MBP), natriuresis, glomerular filtration rate (GFR), renal plasma flow (RPF) and plasma aldosterone level were established. The following were studied: expressions of total Na+,K+,-ATPase (t-NKA); dephosphorylated NKA (d-NKA); cytochrome P4504A (CYP4A); Na+K+-2Cl- (NKCC2) and Na+/Cl- (NCC) cotransporters. The mRNA expression of the NKA α1 (Atp1a1) chain was examined through PCR analysis in the renal cortex and marrow. Results: Male rats having both types of diet showed higher MBP and lower natriuresis. High sodium intake triggered lower aldosterone levels in both sexes; GFR was lower in females and RPF was higher in males (4.09 ± 0.17 vs. 2.81 ± 0.12 ml/min/gr; p<0.01 vs. diet with a normal sodium level). Marrow t-NKA, d-NKA and Atp1a1 were higher in males on both diets. High sodium intake caused lower marrow t-NKA as well as lower cortex and marrow d-NKA in females. In the case of males, only marrow d-NKA decreased. Furthermore, females showed a higher level of CYP4A and lower levels of NKCC2 and NCC, whereas males showed higher levels of NKCC2 and no variations in NCC. Conclusion: Sex conditions blood pressure and sodium balance, reducing resorption in females and increasing RPF in males. This suggests the possibility of studying sodium metabolism disorders differently according to sex.

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Published
2018-03-01
How to Cite
1.
Di Ciano LA, Azurmendi PJ, Vlachovsky SG, Oddo EM, Arrizurieta E, Silberstein CM, Ibarra FR. Gender differences in blood pressure, renal function and response to a high-sodium diet in Wistar rats. Rev Nefrol Dial Traspl. [Internet]. 2018Mar.1 [cited 2024Nov.24];38(1):15-7. Available from: http://revistarenal.org.ar/index.php/rndt/article/view/294
Issue
Vol. 38 No. 1 (2018): Ene.-Mar.
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Original Article